Figure 7. Secretagogue stimulated cAMP accumulation is enhanced by bicarbonate treatment and is inhibited by the sAC inhibitor KH7.

Acini were treated with or without either the sAC inhibitor KH7 [25 µM] for 2 hours. During the last 15 min, IBMX was added [1 mM]. Media was changed and inhibitors where re-added when appropriate. Media used for the change contained either bicarbonate [25 mM] at a constant flow of air/CO₂ (95/5%) or no added bicarbonate under room air conditions. Secretagogue, CER 100 nM (A) or CARB 1 mM (B) was added immediately after media change to the appropriate wells and acini incubated for 1 hour. Acini were collected and assayed for cAMP accumulation. *p<0.05 comparing treatments with and without bicarbonate, #p<0.05 vs corresponding treatment without KH7, @ p<0.05 vs corresponding control without secretagogue. (n≥4).