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Acini were treated with or without bicarbonate (25 mM) either alone or in the presence of either CER 100 nM or CARB 1 mM for 2 hours. Cells were collected and a cell free fraction obtained. Both the cell-free and cell-containing fractions were assayed for LDH and the results expressed as the percentage of LDH released into the media. (n≥3).
