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In this series of experiments 5 µM of PtxR was titrated with 3.2 µl aliquots of a 50 µM solution containing the PtxR operator region of PtoxA. Upper panel: Raw titration data. (A) Titration of PtxR with buffer. (B) Titration of PtxR with DNA. Lower panel: Integrated and dilution corrected raw data. Data were fitted with the “One binding site model” of the MicroCal version of ORIGIN.
