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. 2012 Jul 23;7(7):e41355. doi: 10.1371/journal.pone.0041355

Figure 2. TBX2 promotes migration and invasion of mammary epithelial cells.

Figure 2

(A, B) In vitro ‘scratch’ assays (see Methods) monitoring the migration of (A) murine HC11 and (B) human MCF10A cells stably expressing pCDNA3 vector (+vector) or pCDNA3-TBX2 (+TBX2) over a period of 24–32 hours (h). Representative bright field images of cells (10x magnification) are shown in the left panel. Right panel: statistical evaluation of the distance between the two borders (dotted lines; left panels) at different time points after ‘scratch’ (n = 3; ANOVA test). (C, D) Transwell matrigel invasion assays show a significantly increased ability of TBX2-expressing HC11 (C) and MCF10A cells (D) to invade through a matrigel layer (n = 3, Student’s t-test). The mean ± S.D. is shown. P values are indicated.