Figure 5. MSC-derived Gal-1 promotes tumor metastasis.

Lungs were isolated from mice treated as described under Fig. 3. and fixed with formaldehyde. The lungs were weighed (A upper panel) and the metastatic nodules (marked with black arrows) were counted (A lower panel). * p<0.05, ** p<0.01, n = 5. Control mice were injected with either PBS, wtMSC or MSC^Gal-1−/−. (B) Micro-metastases were evaluated on paraffin-embedded, haematoxylin-eosin-stained lung sections. Metastases (m) are delineated by black arrows. Percentage of lung metastatic area (lower graph) was determined as described in Materials and methods. Scale bar: 1 mm.