FIGURE 1.
Luminex™ validation by single-analyte ELISA. To investigate the reliability of the screening multiplex (Luminex™) assays, individual ELISA assays were performed for VEGF and IL-6, using the same sera as for Luminex. Luminex™ results were highly correlated with single analyte ELISA for VEGF, and moderately correlated with IL-6. In addition, VEGF and IL-6 levels were highly correlated with each other in individual patients’ sera (p<0.001, Spearman correlation coefficient 0.832).