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. 2012 Jul 23;7(7):e41330. doi: 10.1371/journal.pone.0041330

Figure 2. PIN lesions are rapidly reversed by doxycycline withdrawal.

Figure 2

(A,B) Adult double transgenic mice or WT control mice were untreated or treated for 5 weeks with doxycycline and pairs of mice were then taken off of doxycycline for 0–5 days and sacrificed. Protein extracted from half of each ventral prostate was immunoblotted for pAKT (S473 or T308 where indicated), HA, and β-tubulin (loading control). (C) Histology of representative sections of ventral prostate after 0–5 days of doxycycline withdrawal. (D) Immunostaining of representative ventral prostate sections for pAKT (S473), pS6, and cleaved caspase-3. (E) Mean % cleaved caspase 3 positive epithelial cells counted in ten random fields from two mice at each time point. (F) Cleaved caspase 3 immunoblot of proteins extracted from ventral prostate in mice on doxycycline or 1 day after doxycycline withdrawal.