Figure 4. p27 expression is rapidly induced by doxycycline prior to development of PIN.

(A) p27 immunostaining in ventral prostate of double transgenic mice treated for 8 weeks with or without doxycycline, and in prostate from a nontrangenic normal control (higher power). (B) p27 and pAKT expression in double transgenic ventral prostate after 2 weeks of doxycycline. (C) pAKT and p27 expression in ventral prostate of adult double transgenic mice treated with doxycycline for 2, 4, or 7 days. Sections shown are representative of ventral prostates from at least 3 mice at each time.