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. 2012 Jul 23;7(7):e41260. doi: 10.1371/journal.pone.0041260

Figure 2. N. gonorrhoeae does not impact OVA uptake and processing by BMDCs, but does induce maturation and co-stimulatory molecule expression and inflammatory cytokine/chemokine production on BMDCs.

Figure 2

A) Flow cytometric analysis of DQ-OVA endocytosis by BMDCs exposed to N. gonorrhoeae versus control (MOI = 1). B) Gating strategy of BMDCs used for surface marker expression analysis (B220− CD11c+). C–F) Representative histograms from 3–4 independent experiments showing BMDC expression of CD80, CD86, CD40 and MHC class II 24 hours post stimulation with medium only, OVA (100 µg/mL), or N. gonorrhoeae (MOI = 1, 10) with OVA (100 µg/mL). BMDCs were exposed to N. gonorrhoeae at MOI of 1 or 10, alone or with OVA and cytokine and chemokine secretion was determined using multiplex bead-based assay analysis of the culture supernatants as described in the experimental procedures. G) KC, H) MIP-1β Data shown are mean ± standard deviation (N = 4).