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. 2012 Jul 23;7(7):e41109. doi: 10.1371/journal.pone.0041109

Figure 3. Histamine immunofluorescence in the medulla and lobula complex.

Figure 3

A,B: Horizontal section, double-labeled with anti-histamine (magenta in A,B) and anti-synapsin (green in B) antibodies. A large histamine-positive neuron with cell body near the anterior rim of the medulla (Me) extends its axon into the second optic chiasma (2.OC). Proximal layers 2 and 3 of the lobula (Lo), but not layer 1 are densely innervated by processes from the central brain (double arrow in B). A few processes in the lobula pass through layer 1 and invade the outer layer of the lobula plate (LP, arrowhead in A). Double arrowheads indicate thin fibers, connecting the lobula plate to the proximal surface of the medulla. The accessory medulla (aMe) is free of labeling. In medulla layer 5, fine fibers parallel to columns are labeled (arrows in A). C: Frontal section through the medulla, showing three immunoreactive cell bodies at the anterior rim of the medulla (arrowheads; dorsal to the left and distal to the top). Their processes project to the proximal surface of the medulla (Me) and ramify into numerous fine fibers that innervate layers 6–8 of the medulla. Asterisk in A indicates stemmata. Lo, lobula. Scale bar = 100 µm in B (applies to A),C.