Table 1. Antibodies used for immunocytochemistry.
Antibody | Immunogen | Working dilution | Source |
Anti-histamine | histamine coupled to KLH with carbodiimide | 1∶500 (fluorescence) | Cat. No. AB5885, Chemicon-Millipore |
Anti-serotonin (5-HT) | 5-HT coupled to BSA with PFA | 1∶1,000 (fluorescence); 1∶200,000 (PAP) | Cat. No. 20080, ImmunoStar |
Anti-tyramine | p-tyramine-glutaraldehyde-N-alpha-acetyl-L-lysine-N-methylamide | 1∶1,000 (fluorescence); 1∶5,000 (PAP) | Cat. No. AB124, Chemicon-Millipore |
Anti-GABA | GABA coupled to BSA | 1∶1,000 (fluorescence) | Cat. No. A 2052, Sigma |
Anti-synapsin | synapsin protein coupled to GST | 1∶50 to 1∶80 (fluorescence) | 3C11, DSHB |
Abbreviations: BSA, bovine serum albumin; DSHB, Developmental Studies Hybridoma Bank; GABA, γ-aminobutyric acid; GST, glutathione-S-transferase; KLH, keyhole limpet hemocyanin; PAP, peroxidase-anti-peroxidase technique; PFA, paraformaldehyde.