Figure 5. Respiratory LPS exposure does not increase the rate of proliferation of vaccine-induced airway luminal T cells.

(A) Experimental schema; At 14 days post-LPS or -PBS delivery or 1 day post-CFSE delivery, mice were sacrificed and BAL cells were isolated, and Ag85A tetramer (Tet)-specific CD8 T cells (B) were determined by immunostaining and FACS. Representative dotplots are shown with the average frequency ± SEM from three animals/group. The Ag85A tetramer-specific CD8⁺ T cells were further analyzed for the rate of proliferation based on the extent of dilution of incorporated fluorochrome CFSE. The representative histograms of CFSE dilution profiles of BAL Tet⁺CD8⁺ T cells generated by using FlowJo software are shown (C left). The average percentages ± SEM from three animals/group of BAL tet⁺CD8⁺ T cells in various generations (G) of cell division are also shown (C right). Data are representative of two independent experiments.