Figure 7. Respiratory LPS exposure-increased airway luminal T cells in the lung of vaccinated animals is dependent on the recruitment of peripheral T cells.

(A) Experimental schema; At 1 day after each FTY720 delivery, the deficiency of peripheral blood T cells was verified. The representative histogram is shown (B). At 5 days post-last FTY720 or -vehicle delivery, mice were sacrificed and BAL and lung cells were isolated, and Ag85A tetramer (Tet)-specific (C) and IFN-γ-secreting (D) CD8⁺ T cells in BAL or lung interstitium were determined by immunostaining, intracellular cytokine staining and FACS. Representative dotplots are shown with the average frequency ± SEM from three animals/group. The data in graphs are expressed as mean value ± SEM of three animals per group, representative of two independent experiments.