Figure 7.

Radioligand competition binding assays of sorafenib. Ki value is calculated as: Ki = IC₅₀/(1+L/Kd) (Cheng-Prusoff equation), in which [L] = the radioligand concentration used in the binding assay and Kd is the affinity of radioligand at corresponding receptor. [³H]-Ketanserin was used for 5-HT_2A binding; [³H]-LSD for 5-HT_2B and 5-HT_2C binding. For 5-HT_2A, [L] = 3.54 nM and Kd = 2.2 nM; for 5-HT_2B, [L] = 2 nM and Kd = 0.5 nM; for 5-HT_2C, [L]= 2 nM and Kd = 0.6 nM.