Table 5.
RT‐PCR analysis of oral swabs from ruddy turnstones experimentally inoculated with highly pathogenic avian influenza isolate A/whooperswan/Mongolia/244/05 (H5N1)
| Bird ID | Dose* | DPI 0 | DPI 1 | DPI 2 | DPI 3 | DPI 4 | DPI 5 | DPI 6 | DPI 7 | DPI 8 | DPI10 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 67 | Control | –** | – | – | – | – | – | – | – | – | – |
| 79 | Control | – | – | – | – | – | – | – | – | – | – |
| 64 | 4·25 | – | 26·9*** | 33·46 | 36·38 | – | 38·75 | –c | – | – | – |
| 12 | 4·25 | – | 31·26 | 28·01*** | 34·55 | 33·37 | 27·83*** | 31·49 | 37·13 | – | 38·21 |
| 63 | 4·25 | – | – | – | 30·31*** | 31·13 | 32·51***c | 33·81 | 36·59 | 34·79 | 38·09 |
| 8 | 4·25 | – | – | – | – | 38·66*** | – | – | – | 39·89 | – |
| 6 | 4·25 | – | 29·97 | 30·05 | 33·29c | 33·56c | 29·28c | 28·81 | 31·86*** | 34·84 | – |
| 30 | 4·25 | – | ‐ | 37·36 | – | – | – | – | – | – | – |
| 54 | 5·25 | – | 37·28 | 34·12 | – | 39·49 | – | – | – | – | – |
| 65 | 5·25 | – | – | – | – | 37·44 | – | – | – | – | – |
| 52 | 5·25 | – | 31·75 | 37·04 | – | – | – | – | – | – | – |
| 51 | 5·25 | – | 36·75 | 36·61 | 38·89 | – | – | 39·55 | – | – | – |
| 20 | 5·25 | – | 32·77 | 32·78 | 36·54 | – | – | – | – | – | – |
| 16 | 5·25 | – | 27·2*** | 31·26 | 37·15 | 38·77*** | – | – | – | – | – |
| 11 | 6·25 | – | 30·49 | 32·01 | 35·84 | 39·79 | 37·56 | – | – | – | – |
| 9 | 6·25 | – | 27·08*** | 35·63 | 36·3 | 38·74 | – | – | – | – | – |
| 7 | 6·25 | – | 34·51 | 35·22 | 37·93 | – | – | – | – | – | – |
| 70 | 6·25 | – | 27*** | 30·42 | 33·61 | 33·16 | 33·88 | 27·27 | – | – | – |
| 5 | 6·25 | – | 37·27 | – | 37·28 | – | – | – | – | – | – |
DPI, days post‐inoculation.
*Inocula doses expressed as Log10 EID50/100 μl. No bird inoculated with lower viral titers became infected based on viral excretion and seroconversion (not shown).
**C t values are from RT‐PCR analyses using avian influenza H5 specific primers and probe. Samples with no C t values are shown with –. cIndicates that RT‐PCR positive cloacal swabs were detected on the DPI shown.
***Presence of viable virus confirmed by virus isolation in embryonating egg culture.