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. Author manuscript; available in PMC: 2013 Jul 20.
Published in final edited form as: Cell. 2012 Jul 12;150(2):366–376. doi: 10.1016/j.cell.2012.05.016

Figure 4. Isolation of beige precursor cells from inguinal stroma with beige-selective cell surface markers.

Figure 4

Total RNA was isolated from differentiated cultures of beige-selective marker CD137 (A) and TMEM26 (B) positive and negative cells purified from inguinal SV cells; these adipocytes were then assayed for mRNA levels of general adipogenic markers, Ucp-1 and other characteristic thermogenic/brown fat-like genes by qPCR. (C) Primary inguinal SV cells were purified as in (A) and differentiated into adipocytes for 6 days in the presence of FC fragment of human IgG (hFC) (100nM), fusion protein of irisin-FC (100nM) or recombinant FNDC5 (20nM). Total RNA was isolated and assayed for mRNA levels of general adipogenic marker aP2, thermogenic gene Ucp-1 and other brown-like genes by qPCR. Values are mean±SD (n=3). *, p<0.05, **, p<0.01, ***, p<0.001. Similar results were observed in more than three independent experiments.