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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Biochem Pharmacol. 2012 May 10;84(4):486–497. doi: 10.1016/j.bcp.2012.04.021

Fig. 8.

Fig. 8

Nrf-2 is necessary for nitrone cytoprotection. (A) BAEC were transfected with a specific siRNA-Nrf-2 or a scramble siRNA-Control and efficiency of silencing was evaluated by immunoblotting 48 h post-transfection. The results are a representative of three experiments (n = 3). (B) Cytoprotective properties of nitrone was evaluated by MTT assays in BAEC transfected with siRNA-Nrf-2 or siRNA-Control treated 48 h post-transfection with 50 µM nitrone for 24 h followed by treatment with 100 µM H2O2 or diluent control for 24 h. Results are represented as mean ± SEM (n = 3), *p < 0.05 siRNA-Control NT vs. siRNA-Nrf-2 NT and #p > 0.1 siRNA-Control H2O2-treated vs. siRNA-Nrf-2 H2O2-treated, PBN + H2O2 or DMPO + H2O2-treated cells.