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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Fungal Genet Biol. 2012 Jun 9;49(8):619–625. doi: 10.1016/j.fgb.2012.05.012

Figure 2. Schematic of the fluorescence-based recombination reporter system.

Figure 2

Wild type green fluorescent protein (GFP) or a mutant variant (5′GFP) are fused to histone H1. (Cen. = centromere; Grey oval = green fluorescence; white oval = no fluorescence). Some of the expected octad types are illustrated: 1 - normal Mendelian segregation with no recombination (first division segregation); 2 – Normal Mendelian segregation with a crossover between GFP and the centromere (second division segregation); 3 - conversion of 5′GFP to wild-type; 4 - conversion of wild-type GFP to 5′GFP; 5 & 6 - post-meiotic segregation of uncorrected heteroduplex. 5 and 6 show some of the unusual patterns characteristic of a failure of mismatch repair; 5+:3M in 5 and aberrant 4+:4M in 6.