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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Fungal Genet Biol. 2012 Jun 9;49(8):619–625. doi: 10.1016/j.fgb.2012.05.012

Figure 3. The system can be used to determine the timing of recombination.

Figure 3

In crosses of strains carrying different mutants of hH1::GFP (3′GFP and 5′GFP) as alleles, chromosomes in most asci will not fluoresce (1 in A, B & C). However, occasionally recombination will generate a wild-type GFP allele and fluorescence will become visible (2 in A, B & C). If recombination occurs with normal timing, fluorescence should be observed in young asci (A) and in all subsequent stages. Where the timing of recombination is abnormal, fluorescence will be restricted to a later stage (B & C). With this assay, recombination can also be detected in asci lacking a full complement of spores (C).