Figure 6. Recombination in a cross heterozygous for 5′GFP and 3′GFP mutant alleles.

In this spread of two rosettes, two asci have experienced conversion of mutant GFP to wild-type, resulting in two fluorescent spores in each ascus (2+:6M). The asci in which recombination has occurred have been enlarged to assist the reader. This photomicrograph is of rosettes from a cross heterozygous for 5′GFP and 3′GFP, inserted at his-3.