Table 3.

Reversion of either the 5′GFP or 3′GFP construct inserted at his-3 is very rare compared to allelic recombination frequency. Where fluorescence was not detected, the frequency given is the upper estimate at the 95% confidence level. Fl indicates the frequency of fluorescent events per 10⁵ asci.

Cross	Construct	Asci	GFP+	Fl
T12437 × T12515	3′GFP	27455	0	<1.36
T12498 × T12759	5′GFP	16446	0	<2.28
T12498 × T12515	5′GFP × 3′GFP	13490	*46	371± 50

^*Asci with GFP⁺ spores were as shown:

• 2+:6M - 34
• 1+:7M - 8
• 4+:4M - 4