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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Dev Biol. 2012 May 8;368(2):181–192. doi: 10.1016/j.ydbio.2012.04.034

Figure 3. Misexpression of Ken in the CySC lineage causes an accumulation of CySC- and GSC-like cells.

Figure 3

(A-D) Confocal section through Ken misexpressing testis. (A) Testis immunostained with anti-Vasa (red), anti-ZFH1 (green), and DAPI (blue). Ken misexpression in the CySC lineage leads to excess ZFH1-positive somatic cells, which are displaced from the hub. (B) Testis immunostained with anti-Vasa (red), anti-1B1 (green), and DAPI (blue). Ken misexpression in the CySC lineage causes early germ cells to accumulate throughout the testis. These germ cells are characterized by a dot or dumbbell-shaped fusome usually found only in GSCs and GBs in wild-type testes. (C, D) Testes immunostained with anti-Vasa (red), anti-Tj (green), and pH3 (blue). (C) Ectopic somatic cells cycle as single cells (Tj+/pH3+) displaced far from the hub. Note the cytoplasmic projections that a cycling CySC (arrowhead) forms around a GSC-GB pair. Breakdown of the nuclear membrane during mitosis in this cell causes the nuclear localization of Tj to accumulate in the cytoplasm. (D) Ectopic early germ cells only cycle as single cells (reminiscent of GSCs or GBs) or as GSC-GB-like pairs. Scale bar, 20 m.