Figure 5. ken-mediated CySC and GSC self-renewal is not due to ectopic JAK-STAT pathway activation.
(A, B) Confocal sections through testes immunostained with anti-Stat92E (STAT, green), anti-Vasa (red), and DAPI (blue). (A) In positive control testes with sustained hopTumL expression in the CySC lineage, Vasa-negative somatic cells far away from the hub (not shown) express elevated levels of Stat92E due to ectopic JAK-STAT pathway activation. (B) In testes misexpressing ken in the CySC lineage, Stat92E expression is not detected above background levels in ectopic CySCs or GSCs. Scale bar, 20 m.
