Table 1. ken is necessary for the maintenance of CySCs, but not GSCs.
Days ACI | ken allele | Number of testes with one or more clones/total testes |
|||
---|---|---|---|---|---|
GSC | CySC | SGa | CCb | ||
no hs control | Wild-type | 6% (36) | 0% (36) | 8% (36) | 0% (36) |
1 | 0% (17) | 0% (17) | 0% (17) | 0% (17) | |
02970 | 0% (20) | 0% (20) | 0% (20) | 0% (20) | |
k11035 | 0% (17) | 0% (17) | 0% (17) | 0% (17) | |
n.s | n.s | ||||
| |||||
2 | Wild-type | 59% (51) | 33% (51) | 86% (51) | 57% (51) |
1 | 49% (45) | 49% (45) | 71% (45) | 76% (45) | |
02970 | 57% (49) | 53% (49) | 80% (49) | 71% (49) | |
k11035 | 34% (47) | 55% (47) | 60% (47) | 60% (47) | |
n.s | n.s | ||||
| |||||
6 | Wild-type | 54% (57) | 30% (57) | 56% (57) | 33% (57) |
1 | 39% (44) | 5% (44) | 41% (44) | 11% (44) | |
02970 | 48% (31) | 16% (31) | 55% (31) | 19% (31) | |
k11035 | 56% (25) | 8% (25) | 60% (25) | 16% (25) | |
n.s | P < 0.01 | ||||
| |||||
10 | Wild-type | 53% (43) | 26% (43) | 53% (43) | 30% (43) |
1 | 39% (31) | 0% (31) | 39% (31) | 0% (31) | |
02970 | 54% (26) | 15% (26) | 54% (26) | 15% (26) | |
k11035 | 25% (44) | 2% (44) | 25% (44) | 2% (44) | |
n.s | P < 0.001 | ||||
| |||||
14 | Wild-type | 39% (79) | 14% (79) | 39% (79) | 19% (79) |
1 | 33% (73) | 4% (73) | 34% (73) | 10% (73) | |
02970 | 37% (63) | 5% (63) | 38% (63) | 5% (63) | |
k11035 | 26% (38) | 3% (38) | 26% (38) | 3% (38) | |
n.s | P < 0.05 |
SG, spermatogonial clone;
CC, cyst cell clone; n.s., not significant (Chi square and Fisher’s exact test).