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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Am J Transplant. 2012 Apr 17;12(8):2088–2097. doi: 10.1111/j.1600-6143.2012.04053.x

Figure 1.

Figure 1

Reactivity of immortalized B cell clones to HLA and MICA molecules. The reactivity to HLA and MICA molecules of the immortalized B cell clones was measured by Luminex with an HLA mixed beads kit using the clones supernatant and anti-IgG or anti-IgM secondary antibodies (upper and lower panel, respectively). Clone 4G10 supernatant, which contains IgG and IgM anti-Class I antibodies was further tested by Luminex using Class I single antigen beads. The 20 Class I alleles towards which 4G10 antibodies reacted the most are depicted (right panels). Results are presented after normalization as fold increase compared to control uncoated bead. HLA Class I alleles expressed by the donor are marked with an asterisk.