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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Am J Transplant. 2012 Apr 17;12(8):2088–2097. doi: 10.1111/j.1600-6143.2012.04053.x

Figure 2.

Figure 2

Reactivity of immortalized B cell clones to self-antigens. (A) The reactivity of the 107 B cell clones towards generic self antigens (dsDNA and insulin) as well as towards a whole protein lysate prepared from the kidney cell line HEK293 was measured by ELISA. Results are presented after normalization as fold increase compared to the reference value. (B) Reactivity of the 4 selected immortalized clonal supernatants to permeabilized HEp-2 cells. Staining of HEp-2 cells was revealed using a FITC-conjugated anti-IgG/M secondary antibody. All pictures were acquired using the same settings for consistency purposes.