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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Am J Transplant. 2012 Apr 17;12(8):2088–2097. doi: 10.1111/j.1600-6143.2012.04053.x

Figure 3.

Figure 3

Immunoglobulin heavy chain variable region sequence analysis of selected immortalized clones. (A) The uniquely rearranged Ig VH sequences of the 3 selected polyreactive B cell clones are aligned with their closest germline variable sequence (IMGT/V-Quest [34]). The complementary determining regions 1–3 are highlighted in grey. The table reports the comparison between the clonal sequences and the nearest germline sequence. (B) The expression of the B cell memory marker CD27 at the surface of the 3 selected polyreactive B cell clones was assessed by flow cytometry. Filled gray histograms show the signal generated by staining with the corresponding isotype control.