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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Dev Biol. 2012 Jun 7;368(2):312–322. doi: 10.1016/j.ydbio.2012.05.034

Fig. 1. The Na,K-ATPase is required for brain ventricle development.

Fig. 1

(A–F) Loss of function Na,K-ATPase embryos brightfield dorsal view images of wild type (A), partial loss of atp1a1 with mutants snkto273a (B) and hadm883 (C) and splice site MO (D) or severe loss of atp1a1 with start site MO (E) and loss of function fxyd1 splice site MO (F). (G–R) Neuroepithelial formation in Na,K-ATPase morphants/mutants labeled with Zo-1 (G–L) or aPKC (green) and actin (phalloidin; red) (M–R). Images taken at 24 hpf with anterior to the left. (S) Experimental design. Red line indicates region of transverse section in 12 hpf embryo next to corresponding model of transverse section with apically localized junctions (green lines). (T–V) Transverse vibratome sections of 12 hpf embryos stained with Zo 1 (green) and propidium iodide (red) in control (T) atp1a1 start site MO (U), and fxyd1 MO (V). (T’–V’) Zo 1 only. Arrow indicates neuroepithelial apical junction complex. OV = optic vesicle. Asterisk = ear, F = forebrain, M = midbrain, H = hindbrain. Scale bars = 50µm.