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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Dev Biol. 2012 Jun 7;368(2):312–322. doi: 10.1016/j.ydbio.2012.05.034

Fig. 6. Constitutively active RhoA rescues neuroepithelial formation.

Fig. 6

(A–H) Constitutively active RhoAV14 (C,D,G,H,K,L,O,P), or control mRNA (GFP; A,B,E,F,I,J,M,N) in control MO (A–D), atp1a1 start site MO (E–H), fxyd1 MO (I–L), and snkto273a (M–P). (Q–R) Overexpression of dominant negative RhoAN19. (S–T) Dye retention assay in wild type embryos expressing control mRNA (blue), or RhoAV14 (red) (S) and sibling (sib) + control mRNA (red), snkto273a + control mRNA (blue), sib + RhoAV14 (orange), and snkto273a + RhoAV14 (green) (T). Brightfield dorsal (A–Q) and lateral (A’–Q’) views. Ventricle injection of 2000 kDa Rhodamine Dextran dye (A,C,I,K,Q). Zo −1 (B,D,F,H,J,L,N,P,R). All images taken at 24 hpf with anterior to left. F = forebrain, M = midbrain, H = hindbrain. Asterisk = ear. Scale bars = 50µm.