Figure 4. ADAM13 function is not cell autonomous.

Embryos were injected in one cell of two-cell stage embryos with both GFP mRNA and morpholino against ADAM13 and 19 (GFP+2MO) or with RFP mRNA. At stage 15, CNC expressing the lineage tracer were dissected and grafted into non-injected embryos in either one of the following configurations. Either the RFP expressing explant was grafted ventrally to the morphant crest (Wt leader and 2MO follower) or the RFP expressing crest was grafted dorsally to the morphant crest (Wt follower and 2MO leader). Pictures represent a typical example of each type of grafted embryo before (stage 15) and after migration (stage 26). The histogram represents the percentage of failed migration of each type of CNC (Wt or 2MO) for each grafting configuration. Controls (Ctl) correspond to the graft of GFP expressing CNC alone. Student t-tests between the 2MO CNC grafted alone and all other experimental cases were performed. The experiments show that the co-grafting of wild type CNC significantly rescues morphant CNC migration. The configuration of the graft has no impact on the efficiency of the rescue. The co-grafted morphant CNC had no effect on the ability of wild type CNC to migrate. The histogram represents the average of 7 independent experiments. The number of embryos scored was as follows: Ctl; n=42, 2MO alone; n=40, 2MO leader + Wt follower; n=38; 2MO follower + Wt leader; n=33 n= 47. The error bar represents standard deviation from the mean. * = Statistically different from 2MO alone (p<0.05).