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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: Dev Biol. 2012 May 15;368(2):214–230. doi: 10.1016/j.ydbio.2012.05.005

Fig. 6.

Fig. 6

Widespread Crx>Math5 expression does not extend the profile of RGC births, but decreases the numbers of early-born photoreceptors. (A) Birthdating of Crx>Math5-IRES-Cre transgenic (Crx>Math5 Tg) and non-Tg littermate retinas. Embryos were exposed to BrdU at E15.5 and analyzed at P22. There were 2-fold fewer E15.5 birthdated cells in the ONL, representing rod (arrow) and cone (arrowhead) photoreceptors in Crx>Math5 transgenic animals compared to controls, and corresponding increases in the INL and GCL. (B) Crx>Math5 Tg pups were similarly exposed to BrdU at P1 and harvested at P22. Few, if any, GCL cells were labeled with BrdU in transgenic or control mice. (C) Crx>Math5-IRES-Cre BAC (BAC) embryos were exposed to EdU at E17.5, and their RGCs were labeled with rhodamine dextran at P22. No EdU+ RGCs were detected in central flatmounts of BAC or control retinas. Prolonged transgenic expression of Math5 does not extend the RGC birthdating profile. Scale bar: 100 µm in A–B; 50 µm in C.