Fig. 4.

Single plane confocal images of fungiform (A–C) and circumvallate (D–I) papillae in Wnt1-Cre/ZEG mouse tongue at P1. EGFP-positive cells (green) are seen, though infrequently, in the epithelium of taste papillae and within early taste buds (white arrows). In G–I, double labeling of GFP and epithelial cell marker E-cadherin (E-cad, red) was performed to confirm location of the labeled cells in epithelial cells. White dotted lines bracket early taste buds. Scale bars: 20 μm.