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Inhibition of colchicine binding, measured by fluorescence, by PAB (○) and podophyllotoxin (●). Fluorescence study was performed following the procedure described by Bhattacharyya and Wolff [4]. Reaction mixtures contained 1 μM tubulin, 5 μM colchicine, and PAB or podophyllotoxin at the indicated concentrations. The symbol △ represents the fluorescence observed with only colchicine in the reaction mixture.
