Figure 1. PFD and dPFD inhibit acute allograft rejection.

Mouse lung transplants were performed and evaluated after 7 days. The different groups include untransplanted mouse lungs (Control), Isografts (C57BL/6 donor lung into C57BL/6 recipient), Allograft (BALB/c lungs transplanted into C57BL/6 mice treated with vehicle), PFD (400mg/kg/day) treated recipient, and dPFD (150 mg/kg/day) treated recipient. Each group represents 4–8 mice per experimental group and significance is based on p value < 0.05. A) Representative in vivo micro CTs of mouse thorax for a control, isograft, allograft, and PFD treated allograft-showing improvement in PFD and dPFD treated recipients as compared to untreated recipients. B) PawP (cmH₂O) was significantly reduced in both the PFD and dPFD treated mice as compared to untreated allografts and no different from control or isografts. C) Representative gross pictures of both explanted naive right and left allograft lungs from the different groups with the allograft being hyperemic and smaller as compared to the other groups. D) Representative example of HE stains for each group with allograft showing an increased perivascular and peribronchial inflammatory infiltrate with both PFD and dPFD showing less cellular infiltrate. E) Graphic representation of ACR grade for the different groups with both PFD and dPFD recipients having a significant reduction of ACR as compared to vehicle treated allograft.