Figure 7.
The DCN in the human. A. CV staining. There is an outer layer (1) with few stained somata, a darker band below it (2) with scattered, stained, large somata, and lighter staining and more scattered profiles deep to that (3). The boxed region is shown at higher magnification in B. Scale bar=1 mm. B. Large stained profiles; somata are elongated but not all oriented the same way. Note the many small stained somata (arrowhead). Scale bar=50 μm. C. Laminar organization as shown by label with an antibody to nonphosphorylated neurofilament protein (NPNFP). There is a darkly stained band made up of somata (example at arrow) and a dense meshwork of stained processes. In layer 1 there one stained neuron and a few stained fibers (example at arrowhead). Scale bar=50 μm. D. Calbindin (CB)-immunoreactivity in fine, beaded fibers (example at arrowhead) that overlap layer 2. Scale bar= 50 μm.
