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. Author manuscript; available in PMC: 2013 Jul 17.
Published in final edited form as: Anal Chem. 2012 Jun 27;84(14):5905–5912. doi: 10.1021/ac300254d

Figure 1.

Figure 1

(A) Base peak chromatograms (left panel) and mass spectra (right panel) of ganglioside standards GM3, GD3 and GT1b. A C18 analytical column was used as reverse-phase mode for the separation of ceramide moieties. 20-50 ng samples were injected. Inserts are the sugar structures of each ganglioside. Right panel mass spectra show gangliosides with ceramide d38:1 from LC separation, which is indicated by arrows on the left panel. Ganglioside subspecies and their assignments are shown in S-Table 1. (B) Retention time vs. m/z plot of the ganglioside standards. Ganglioside separation mainly depends on the hydrophobicity of ceramide moieties.