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. Author manuscript; available in PMC: 2013 Jul 17.
Published in final edited form as: Anal Chem. 2012 Jun 27;84(14):5905–5912. doi: 10.1021/ac300254d

Figure 2.

Figure 2

Separation of complex gangliosides on the nanoHPLC chip. (A) EIC of bovine milk ganglioside GD3 (d41:1) at m/z 769.952. Separation of the monoisotopic peak of NeuAc-NeuAc GD3 (d41:1) and the third isotopic peak of NeuAc-NeuAc GD3 (d41:2) was achieved. The contribution of 13C was removed by the LC. (B) EIC of NeuGc NeuAc GD3 (d40:1) at m/z 770.941. The separation of NeuAc containing gangliosides and NeuGc containing gangliosides was achieved. The first peak and second peak correspond to the monoisotope of NeuGc NeuAc GD3 (d40:1) and the third isotope of NeuAc-NeuAc GD3 (d41:1), respectively. All the ions are doubly depronated. The isotopic peaks of other compounds are indicated by asterisks (*). Inserts are the spectrum at each retention time.