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. Author manuscript; available in PMC: 2013 Jul 17.
Published in final edited form as: Anal Chem. 2012 Jun 27;84(14):5905–5912. doi: 10.1021/ac300254d

Figure 3.

Figure 3

Detection and identification of individual classes of polar lipids in mouse brain extracts. (A) A two dimensional plot of m/z vs. retention time for mouse brain polar lipids. (B) Base peak chromatogram (BPC) from MS analysis, (C) post processing precursor ion scan for m/z 290.095 (gangliosides), (D) for m/z 96.960 (sulfatide), and (E) for m/z 241.011 (phosphatidylinositol). The fragment ions for the individual headgroup being characteristic of their species can be used for identification of their lipids within one class, followed by alignment with the BPC. Gangliosides, which are the most polar lipids, eluted early in the chromatography. EICs with 10 ppm window were used for the scans. Both BPC and EICs were obtained in the negative ion mode. Details are described in the experimental section.