Figure 5. Imaging mass spectrometry analysis of Kenyan WT.
(A) Image guided selection of histologic areas of interest in two Kenyan WT (KWT). To control for differences in peptide spectra between histologic compartments, comparisons were made between specific cell compartments only (e.g., blastema versus blastema; stroma versus stroma). (B) Unsupervised hierarchical clustering of peptide spectra from blastema only. Kenyan (K) WT specimens cluster together and separately from North American specimens (B, Black patient; W, White patient; numbers identify duplicate specimens from same patient WT). (C) Principal component displays two peptides detected in the blastemal compartment that distinguish Kenyan from North American WT.
