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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Microbiol. 2012 Jun 12;85(3):445–460. doi: 10.1111/j.1365-2958.2012.08120.x

Fig. 1.

Fig. 1

Correlation between secretin production and reduced growth yield of a psp null strain

A. YsaC-dependent growth inhibition depends on the amount of YsaC produced. psp+ (AJD3) and Δpsp (AJD1171) strains containing pBAD33 (− YsaC) or pAJD935 (+ YsaC) were grown with the indicated arabinose concentrations. Optical density was measured every hour.

B. Anti-His6 (for YsaC-His6) immunoblot analysis of cells grown as in panel A, harvested at the 3 h time point. Ponceau S stain of the YsaC region of the nitrocellulose membrane is shown as a loading control.

C. YscC-dependent growth inhibition depends on the amount of YscC produced but is alleviated by the pilot protein YscW. psp+ (AJD3) and Δpsp (AJD1171) strains containing pVLT35 (− YscC), pAJD126 (+ YscC), or pAJD136 (+ YscC YscW) were grown with the indicated amounts of IPTG. Optical density was measured every hour.

D. Anti-YscC immunoblot analysis of cells grown as in panel C, harvested at the 3 h time point. Ponceau S stain of the YscC monomer region of the nitrocellulose membrane is shown as a loading control. Multimers and monomers were detected on the same nitrocellulose membrane but the intervening region, which was blank, has been excised.