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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Mol Microbiol. 2012 Jun 12;85(3):445–460. doi: 10.1111/j.1365-2958.2012.08120.x

Fig. 2.

Fig. 2

Evidence that secretins decrease the membrane potential in an E. coli psp null strain but non-secretin Psp-inducers do not. E. coli psp+ (MC3) and Δ(pspF-pspE) (AJDE2419) strains contained pVLT35-based vectors encoding Y. enterocolitica Psp-inducers. For all panels IPTG was added to cultures at 200 μM for empty pVLT35 vector (−) and YscC, none for YsaC, 25 μM for AmpE, and 5 μM for YE0566.

A. Φ(pspA-lacZ) operon fusion expression in the psp+ strain. Cultures were grown as described in Experimental procedures. Error bars represent one positive standard deviation.

B. Growth of the psp+ and Δ(pspF-pspE) stains. Optical density was determined hourly. C. [3H]-TPP+ accumulation by whole cells grown as in panel A. Error bars represent one positive standard deviation. CPM = counts per minute.