Table 1.
Strains and plasmids
| Name | Genotype/Features | Reference or Source |
|---|---|---|
| E. coli K-12 strain | ||
| MC3 | F−
araD139δ(argF-lac) U169 relA1 rpsL150 flbB5301 depC1 ptsF25 rbsR λ[Φ(pspAp-lacZY)] |
Bergler et al., 1994 |
| AJDE2419 | MC3 Δ(pspF-E)::kanr | This study |
| Y. enterocolitica strains | ||
| AJD3 a | ΔyenR (R−M+) Nalr | Laboratory collection |
| AJD977 | ΔyenR (R−M+) ΔaraGFB::[Φ(pspAp-lacZY)] | Maxson and Darwin, 2005 |
| AJD1171 | ΔyenR (R−M+) Δ(pspF-ycjF) ΔpspG | Maxson and Darwin, 2006 |
| AJD3298 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] | Yamaguchi et al., 2010 |
| AJD3463 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔpspB | This study |
| AJD3465 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔpspC | This study |
| AJD3469 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔpspBC | This study |
| AJD3494 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] Δ(pspD-ycjF) | This study |
| AJD3497 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔpspA | This study |
| AJD4250 | ΔyenR (R−M+) ΔaraGFB::[Φ(catp-lacZ)] | This study |
| AJD4251 | ΔyenR (R−M+) Δ(pspF-ycjF) ΔpspG ΔaraGFB::[Φ(catp-lacZ)] | This study |
| AJD4505 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔaraGFB::[Φ(catp-lacZ)] | This study |
| AJD4507 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔaraGFB::[Φ(catp-lacZ)] ΔpspB | This study |
| AJD4508 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔaraGFB::[Φ(catp-lacZ)] ΔpspC | This study |
| AJD4510 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔaraGFB::[Φ(catp-lacZ)] ΔpspBC | This study |
| AJD4511 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔaraGFB::[Φ(catp-lacZ)] δ(pspD-ycjF) | This study |
| AJD4512 | ΔyenR (R−M+) ΔpspF ΔpspAp::[lacIq-tacp] ΔaraGFB::[Φ(catp-lacZ)] ΔpspA | This study |
| Plasmids | ||
| pACYC184 | Cmr Tetr, p15A ori | Chang and Cohen, 1978 |
| pBAD33 | Cmr, p15A ori, araBp expression vector | Guzman et al., 1995 |
| pRE112 | Cmr, R6K ori, mob+ (RP4), sacB+ | Edwards et al., 1998 |
| pSR47S | Kmr, R6K ori, mob+ (RP4), sacB+ | Merriam et al., 1997 |
| pVLT35 | Smr, Spr, RSF1010 ori, tacp expression | vector de Lorenzo et al., 1993 |
| pAJD126 | tacp-yscC in pVLT35 | Darwin and Miller, 2001 |
| pAJD136 | tacp-yscCyscW in pVLT35 | Darwin and Miller, 2001 |
| pAJD555 | tacp-ysaC in pVLT35 | Maxson and Darwin, 2004 |
| pAJD633 | tacp-ampE in pVLT35 | Maxson and Darwin, 2004 |
| pAJD634 | tacp-YE0566 in pVLT35 | Maxson and Darwin, 2004 |
| pAJD935 | araBp-ysaC-his6 in pBAD33 | This study |
| pAJD1806 | tacp-pilQ-his6 in pVLT35 | This study |
| pAJD2049 | Φ(catp-lacZ) in pACYC184 | This study |
a
AJD3 is a virulence plasmid cured derivative of strain JB580v (Kinder et al., 1993). All other Y. enterocolitica strains listed are derivatives of AJD3.