Figure 2. Hydrolase activity assay in cultured neurons from male and female rat brains.

On day 10 in vitro, neuronal cultures from male (M) and female (F) rats were homogenized and incubated with epoxyfluor 7, which, when hydrolyzed by sEH, becomes a fluorescent compound. Fluorescence was measured using a fluorescent plate reader, and activity was normalized to reaction time and amount of protein. (n=6, *p=0.01)