Figure 7. Inhibition of sEH using 4-PCO does not alter the difference in sensitivity to OGD between cells from males vs. females.

On day 10 in vitro, cultured cortical neurons from males and females were treated with 4-Phenylchalcone oxide (4-PCO, 2 µM), an inhibitor of the hydrolase activity of soluble epoxide hydrolase, or vehicle (DMSO). Treatment was initiated 1 hour prior to OGD and continued throughout OGD and 24 hours of reoxygenation. Cells from females were more resistant to cell death in both vehicle and 4-PCO treated cells as expressed relative to the mean in cells from males (n=5, *p<0.05).