Fig. 3.

AtSN1 and AtCOPIA4 DNA methylation in suvh2, suvh9 and suvh2 suvh9 mutants. DNA methylation was determined by bisulfite sequencing of genomic DNA from 7 day old seedlings or leaves of mature 6 week old plants. Individual clones (N) were sequenced for the different genotypes. The bars mark the levels of DNA methylation in percent of methylated cytosines relative to total cytosines, with black indicating all cytosines, dark grey CG context, light grey CHG context and red indicating CHH context. Wt indicates wild type plants. a DNA methylation in AtSN1. Significance of differences in total DNA methylation between the different genotypes were checked by Chi Square tests (table, ***p < 0.001 and ANOVA, data not shown). b DNA methylation at AtCOPIA4