Fig. 4.

AtSN1-derived transcripts in suvh2, suvh9 and suvh2 suvh9 mutants. AtSN1 transcripts were determined relative to ACT2 and PFK mRNA in total RNA from imbibed seeds, 7 day old seedlings or leaves of mature 6 week old plants by RT PCR-based methods with primers specific for transcripts A and B. a Semiquantitative PCR analysis of AtSN1 transcripts A and B in imbibed seeds and leaves in comparison to ACT2 mRNA. b RT-qPCR analysis of AtSN1 transcript A (yellow) and PFK mRNA (grey) in imbibed seeds, leaves and seedlings relative to ACT2 mRNA. The bars indicate the median of 5 biological samples measured in technical duplicate; the error bars indicate the total deviation among samples