Fig. 5.

AtSN1-associated histone modifications in suvh2, suvh9 and suvh2 suvh9 mutants. Chromatin immunoprecipitation combined with quantitative PCR (ChIP-qPCR) was performed on 1 week old seedling with antibodies specific to H3, H3K4me3 and H3K9me2 and primers specific for PFK, AtSN1 regions A, B, C and Ta3. Wild type values were set to 1.0 and values for mutants were calibrated accordingly. Black indicates wild type, light-grey suvh2 single mutants, dark-grey suvh9 single mutants and red suvh2 suvh9 double mutants. Quantification by qPCR was performed in duplicates. Bars indicate the mean; error bars the standard deviation from 3 independent experiments. a H3, b H3K4me3, c H3K9me2