Fig. 1.

Yeast complementation assay. The vectors pDR195 (Empty), pDR196-ZmYS1 (ZmYS1), and pDR195-OsYSL16 (OsYSL16) were introduced respectively into the yeast strain Δfet3Δfet4. Each strain was grown on SD medium. a Fe(III)-DMA (pH 7.0): 20 μM FeCl₃ with 100 μM DMA. b Fe(II)-NA (pH 7.0): 10 μM FeCl₂ with 100 μM NA. c FeCl₃, 0 μM (pH 7.0). d FeCl₃, 20 μM (pH 5.0). The yeast were washed with EDTA, and water was added to adjust the OD₆₆₀ to 1.0, 0.1, 0.01, and 0.001 (from the left) before spotting