Fig. 5.

Early growth of OsYSL16-knockdown plants. a Picture of wild-type rice (WT) and OsYSL16-knockdown seedlings (17i, 21i) grown with different Fe sources. b Shoot length of wild-type plants (WT) and knockdown plants (17i and 21i) on MS medium with different metal nutrition conditions. c Root length. Fe(II)Cl₂: 10 μM Fe(II)Cl₂ in place of 100 μM Fe(III)-EDTA. Fe(III)Cl₃: 10 μM Fe(III)Cl₃ in place of 100 μM Fe(III)-EDTA. –Fe: MS medium without Fe. –Cu: MS medium without Cu. –Zn: MS medium without Zn. –Mn: MS medium without Mn. FeCit: 100 μM Fe(III)-citrate in place of 100 μM Fe(III)-EDTA. *Shows significant differences from WT (p < 0.05) based by Student’s t test (n of WT = 10, n of 17i and 21i = 5); mean ± SD, **Shows significant differences from WT (p < 0.01) by Student’s t test. The plants which did not germinate (when length of root or shoot was less than 1 mm) were not included in the calculation for statistics