Fig. 6.

Growth of OsYSL16-knockdown plants in vegetative stage. Wild-type and knockdown (17i and 21i) plants after 4 (a–c and e–g) and 6 (d, h) weeks in a hydroponic solution containing 100 μM of Fe(III)-EDTA (a–d) and without Fe (e–h) for 1 week. a SPAD value under Fe-sufficient conditions. b Shoot length under Fe-sufficient conditions. c Root length under Fe-sufficient conditions. d Fe allocation into new (first- and second-newest) and old leaves (third-, fourth-, and fifth-newest) under Fe-sufficient conditions. e SPAD value under Fe-deficient conditions. f Shoot length under Fe-deficient conditions. g Root length under Fe-deficient conditions. h Fe allocation into new and old leaves under Fe-deficient conditions. *Shows significant differences from WT (p < 0.05) based by Student’s t test (n of 4-week-old WT = 10; n of 4-week-old 17i and 21i = 5; n of 6-week-old plants = 3); means ± SD